Searchable abstracts of presentations at key conferences in endocrinology

ea0035p519 | Endocrine tumours and neoplasia | ECE2014

BRAF V600E mutation in washing liquid of thyroid fine-needle aspiration: a surprising tool in cytological benign nodules

Monzani Maria Laura , Brigante Giulia , Marino Marco , Bonacini Lara , Pignatti Elisa , Cioni Katia , Madeo Bruno , Rochira Vincenzo , Santi Daniele , Maiorana Antonino , Carani Cesare , Simoni Manuela

Objective: Thyroid fine-needle aspiration (FNA) cytology is indeterminate in 15–25% of cases. Recently, cytological analysis was combined to molecular analysis to improve diagnostic accuracy. In the present study, washing liquid of FNA (wFNA) samples were tested for the BRAF V600E mutation, using high resolution melting (HRM) technology. The aim of this study was to demonstrate whether BRAF mutation analysis is accurate in wFNA and, when combined with cytological analysis...